Isotachophoresis: Theory and Microfluidic Applications.

ABSTRACT: Isotachophoresis (ITP) is a versatile electrophoretic technique that can be used for sample preconcentration, separation, purification, and mixing, and to control and accelerate chemical reactions. Although the basic technique is nearly a century old and widely used, there is a persistent need for an easily approachable, succinct, and rigorous review of ITP theory and analysis. This is important because the interest and adoption of the technique has grown over the last two decades, especially with its implementation in microfluidics and integration with on-chip chemical and biochemical assays. We here provide a review of ITP theory starting from physicochemical first-principles, including conservation of species, conservation of current, approximation of charge neutrality, pH equilibrium o

SUBMITTER: Ramachandran A 

PROVIDER: S-EPMC9373989 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature