A clinical Pseudomonas juntendi strain with bla _IMP-1 carried by an integrative and conjugative element in China.

ABSTRACT:

Objective

To precisely determine the species of a carbapenem-resistant Pseudomonas strain 1809276 isolated from the urine of a Chinese patient and analyze its integrative and conjugative element (ICE) 1276 formation mechanism.

Methods

Single-molecule real-time (SMRT) sequencing was carried out on strain 18091276 to obtain the complete chromosome and plasmid (pCN1276) sequences, and average nucleotide identity (ANI) was used for precise species identification. The ICEs in GenBank with the same integrase structure as ICE 1276 were aligned. At the same time, the transfer ability of bla _IMP-1 and the antibiotic sensitivity of Pseudomonas juntendi 18091276 were tested.

Results

This bacterium was P. juntendi, and its drug

SUBMITTER: Zheng L 

PROVIDER: S-EPMC9374279 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature