Identification and validation of stable reference genes for quantitative real time PCR in different minipig tissues at developmental stages.

ABSTRACT:

Background

Quantitative real time PCR (qPCR) is a powerful tool to evaluate mRNA expression level. However, reliable qPCR results require normalization with validated reference gene(s). In this study, we investigated stable reference genes in seven tissues according to four developmental stages in minipigs. Six candidate reference genes and one target gene (ACE2) were selected and qPCR was performed. BestKeeper, geNorm, NormFinder, and delta Ct method through the RefFinder web-based tool were used to evaluate the stability of candidate reference genes. To verify the selected stable genes, relative expression of ACE2 was calculated and compared with each other.

Results

As a result, HPRT1 and 18S genes had lower SD value, while HMBS and GAPDH genes had higher SD value in all s

SUBMITTER: Song J 

PROVIDER: S-EPMC9374586 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature