Spontaneous Hind Limb Paralysis Due to Acute Precursor B Cell Leukemia in RAG1-deficient Mice.

ABSTRACT: RAG1-deficient mice are a frequently used immunodeficient mouse strain lacking mature lymphocytes. Apart from an elevated risk for infections, no predispositions for diseases of this strain have been described so far. We here report a high incidence of spontaneous pro B cell leukemia resulting in hind limb paralysis in our colony of RAG1-deficient mice. At an age of 7-13 months, animals developed hind limb paralysis and rapid decrease of the overall health condition leading to the need of euthanasia. Histological and flow cytometric analyses as well as micro-computed tomography (micro-CT) scans revealed CD45⁺ CD19⁺ IgM^- cell infiltrates in the spleen, the bone marrow, and the spinal canal. Monthly blood sampling and screening for CD19⁺ blast freq

SUBMITTER: Feifei L 

PROVIDER: S-EPMC9374608 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature