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Single-atom nanozymes catalytically surpassing naturally occurring enzymes as sustained stitching for brain trauma.

ABSTRACT: Regenerable nanozymes with high catalytic stability and sustainability are promising substitutes for naturally-occurring enzymes but are limited by insufficient and non-selective catalytic activities. Herein, we developed single-atom nanozymes of RhN₄, VN₄, and Fe-Cu-N₆ with catalytic activities surpassing natural enzymes. Notably, Rh/VN₄ preferably forms an Rh/V-O-N₄ active center to decrease reaction energy barriers and mediates a "two-sided oxygen-linked" reaction path, showing 4 and 5-fold higher affinities in peroxidase-like activity than the FeN₄ and natural horseradish peroxidase. Furthermore, RhN₄ presents a 20-fold improved affinity in the catalase-like activity compared to the natural catalase; Fe-Cu-N₆ displays selectivity towards the superoxide dismutase-like activity; VN₄ favors a 7-fold higher glutathione peroxidase-like activity than the natural glutathione peroxidase. Bioactive sutures with Rh/VN₄ show recyclable catalytic features without apparent decay in 1 month and accelerate the scalp healing from brain trauma by promoting the vascular endothelial growth factor, regulating the immune cells like macrophages, and diminishing inflammation.

SUBMITTER: Zhang S

PROVIDER: S-EPMC9374753 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature

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Single-atom nanozymes catalytically surpassing naturally occurring enzymes as sustained stitching for brain trauma.

Zhang Shaofang S Li Yonghui Y Sun Si S Liu Ling L Mu Xiaoyu X Liu Shuhu S Jiao Menglu M Chen Xinzhu X Chen Ke K Ma Huizhen H Li Tuo T Liu Xiaoyu X Wang Hao H Zhang Jianning J Yang Jiang J Zhang Xiao-Dong XD

Nature communications 20220812 1

Regenerable nanozymes with high catalytic stability and sustainability are promising substitutes for naturally-occurring enzymes but are limited by insufficient and non-selective catalytic activities. Herein, we developed single-atom nanozymes of RhN4, VN4, and Fe-Cu-N6 with catalytic activities surpassing natural enzymes. Notably, Rh/VN4 preferably forms an Rh/V-O-N4 active center to decrease reaction energy barriers and mediates a "two-sid ...[more]

PMID: 35961961

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Project description:Cytochromes P450 (P450s) and glutathione S-transferases (GSTs) constitute two important enzyme families involved in carcinogen metabolism. Generally, P450s play activation or detoxifying roles while GSTs act primarily as detoxifying enzymes. We previously demonstrated that oral administration of the linear furanocoumarins, isopimpinellin and imperatorin, modulated P450 and GST activities in various tissues of mice. The purpose of the present study was to compare a broader range of naturally occurring coumarins (simple coumarins, and furanocoumarins of the linear and angular type) for their abilities to modulate hepatic drug-metabolizing enzymes when administered orally to mice. We now report that all of the different coumarins tested (coumarin, limettin, auraptene, angelicin, bergamottin, imperatorin and isopimpinellin) induced hepatic GST activities, whereas the linear furanocoumarins possessed the greatest abilities to induce hepatic P450 activities, in particular P450 2B and 3A. In both cases, this corresponded to an increase in protein expression of the enzymes. Induction of P4502B10, 3A11, and 2C9 by xenobiotics often is a result of activation of the pregnane X receptor (PXR) and/or constitutive androstane receptor (CAR). Using a pregnane X receptor reporter system, our results demonstrated that isopimpinellin activated both PXR and its human ortholog SXR by recruiting coactivator SRC-1 in transfected cells. In CAR transfection assays, isopimpinellin counteracted the inhibitory effect of androstanol on full-length mCAR, a Gal4-mCAR ligand-binding domain fusion, and restored coactivator binding. Orally administered isopimpinellin induced hepatic mRNA expression of Cyp2b10, Cyp3a11, and GSTain CAR(+/+) wild-type mice. In contrast, the induction of Cyp2b10 mRNA by isopimpinellin was attenuated in the CAR(-/-) mice, suggesting that isopimpinellin induces Cyp2b10 via the CAR receptor. Overall, the current data indicate that naturally occurring coumarins have diverse activities in terms of inducing various xenobiotic metabolizing enzymes based on their chemical structure.

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Single-atom nanozymes catalytically surpassing naturally occurring enzymes as sustained stitching for brain trauma.

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Single-atom nanozymes catalytically surpassing naturally occurring enzymes as sustained stitching for brain trauma.

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