Absolute protein quantitation of the mouse macrophage Toll-like receptor and chemotaxis pathways.

ABSTRACT: The Toll-like receptor (TLR) and chemotaxis pathways are key components of the innate immune system. Subtle variation in the concentration, timing, and molecular structure of the ligands are known to affect downstream signaling and the resulting immune response. Computational modeling and simulation at the molecular interaction level can be used to study complex biological pathways, but such simulations require protein concentration values as model parameters. Here we report the development and application of targeted mass spectrometry assays to measure the absolute abundance of proteins of the mouse macrophage Toll-like receptor 4 (TLR4) and chemotaxis pathways. Two peptides per protein were quantified, if possible. The protein abundance values ranged from 1,332 to 227,000,000 copies per

SUBMITTER: Manes NP 

PROVIDER: S-EPMC9374760 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature