Methods for Cryosectioning and Mass Spectrometry Imaging of Whole-Body Zebrafish.

ABSTRACT: The zebrafish (Danio rerio) is an ideal model for whole animal studies of lipid metabolism and lipid-related disease. In this work, infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI) mass spectrometry imaging (MSI) was applied for direct visualization of lipid and metabolite distributions across various organs in whole-body zebrafish tissue sections. Detailed methods for overcoming the challenges of cryosectioning adult male zebrafish for MSI and complementary histological imaging are described. Representative two-dimensional ion maps demonstrated organ specific localization of lipid analytes allowing for visualization of areas of interest including the brain, liver, intestines, and skeletal muscle. A high resolving power mass spectrometer was utilized fo

SUBMITTER: Stutts WL 

PROVIDER: S-EPMC9375048 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature