Single-cell transcriptomics reveals the cell fate transitions of human dopaminergic progenitors derived from hESCs.

ABSTRACT:

Background

Midbrain dopaminergic (DA) progenitors derived from human pluripotent stem cells are considered to be a promising treatment for Parkinson's disease (PD). However, the differentiation process produces undesired cell types, which influence the in vivo evaluation of DA cells. In this paper, we analyze the cell fate choice during differentiation and provide valuable information on cell preparation.

Methods

Human embryonic stem cells were differentiated into DA progenitors. We applied single-cell RNA sequencing (scRNA-seq) of the differentiation cells at different time points and investigated the gene expression profiles. Based on the differentially expressed genes between DA and non-DA cells, we investigated the impact of LGI1 (DA enriched) overexpression on DA differ

SUBMITTER: Liang L 

PROVIDER: S-EPMC9375405 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature