MiR-190 ameliorates glucotoxicity-induced dysfunction and apoptosis of pancreatic β-cells by inhibiting NOX2-mediated reactive oxygen species production.

ABSTRACT: Glucotoxicity-induced pancreatic β-cell failure contributes to the development of type 2 diabetes mellitus (T2DM). Accumulating evidence reveals that miRNAs play a critical role in regulating pancreatic β-cell function and survival. In this study, we employed a self-assembled cell microarray (SAMcell)-based functional screening assay to identify miRNAs that are capable of regulating the dysfunction of β-cells induced by glucotoxicity. Among 62 conserved miRNAs we tested, miR-190 was identified as a candidate regulator that could effectively restore insulin expression in NIT-1 cells under high-glucose (HG) stimulation. Further analyses demonstrated that miR-190 was significantly down-regulated in HG-treated NIT-1 cells, as well as in the pancreas of diabetic mice. Mecha

SUBMITTER: Lu H 

PROVIDER: S-EPMC9375543 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature