Treatment of Murine Toxoplasmosis with Oral and Parenteral Artemether and Following by Detection of B1 Gene by Quantitative Real-Time PCR (qPCR) for Evaluating Parasite Density.

ABSTRACT:

Background

Toxoplasma gondii is an obligate intracellular parasite that can infect humans and animals. As the choice drug have shown side effects, development a new drug with low toxicity will be necessary.

Methods

BALB/c mice were infected with tachyzoiets of T. gondii. After treatment by oral and parenteral artemether (250 μg/mice) and sulfadiazine (50 μg/mice), we evaluated the rates of survival in treated and control mice. The fold change of B1 gene (target gene) expression in liver and brain of mice treated with parenteral artemether (i.p.), oral artemether (via gavage) and sulfadiazine, were detected by using the Real-Time quantitative PCR.

Results

Both treatment with sulfadiazine and artemether showed significant prolongation in time to death of t

SUBMITTER: Mikaeiloo H 

PROVIDER: S-EPMC9375721 | biostudies-literature | 2022 Jan-Mar

REPOSITORIES: biostudies-literature