Digital quantitative tissue image analysis of hypoxia in resected pancreatic ductal adenocarcinomas.

ABSTRACT:

Background

Tumor hypoxia is theorized to contribute to the aggressive biology of pancreatic ductal adenocarcinoma (PDAC). We previously reported that hypoxia correlated with rapid tumor growth and metastasis in patient-derived xenografts. Anticipating a prognostic relevance of hypoxia in patient tumors, we developed protocols for automated semi-quantitative image analysis to provide an objective, observer-independent measure of hypoxia. We further validated this method which can reproducibly estimate pimonidazole-detectable hypoxia in a high-through put manner.

Methods

We studied the performance of three automated image analysis platforms in scoring pimonidazole-detectable hypoxia in resected PDAC (n = 10) in a cohort of patients enrolled in PIMO-PANC. Multiple stained tumor

SUBMITTER: Siddiqui I 

PROVIDER: S-EPMC9376475 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature