Exosome-transmitted miR-3124-5p promotes cholangiocarcinoma development via targeting GDF11.

ABSTRACT:

Objective

Cholangiocarcinoma (CHOL) is a deadly cancer worldwide with limited available therapies. The aim of this study was to investigate key exosomal miRNAs and their functions in CHOL development.

Methods

Serum exosomes were isolated from patients with CHOL and healthy controls, followed by miRNA sequencing for identifying differentially expressed miRNAs (DEMs) and their functions. Then, the expression of key DEMs was experimentally validated in exosomes from clinical CHOL patients and CHOL cells. The effects of overexpression of key DEMs on CHOL cell migration and proliferation were investigated. A key exosomal DEM miR-3124-5p was identified. The effects of overexpression or knockdown of exosomal miR-3124-5p on the proliferation, migration, and angiogenesis of human umb

SUBMITTER: Gao H 

PROVIDER: S-EPMC9376483 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature