Hydroxycholesterol substitution in ionizable lipid nanoparticles for mRNA delivery to T cells.

ABSTRACT: Delivery of nucleic acids, such as mRNA, to immune cells has become a major focus in the past decade with ionizable lipid nanoparticles (LNPs) emerging as a clinically-validated delivery platform. LNPs-typically composed of ionizable lipids, cholesterol, phospholipids, and polyethylene glycol lipids -have been designed and optimized for a variety of applications including cancer therapies, vaccines, and gene editing. However, LNPs have only recently been investigated for delivery to T cells, which has various therapeutic applications including the engineering of T cell immunotherapies. While several LNP formulations have been evaluated for mRNA delivery, recent work has demonstrated that the utilization of cholesterol analogs may enhance mRNA delivery. Other studies have shown that cholest

SUBMITTER: Patel SK 

PROVIDER: S-EPMC9376797 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature