Reusable Single Cell for Iterative Epigenomic Analyses.

ABSTRACT: Current single-cell epigenome analyses are designed for single use. The cell is discarded after a single use, preventing analysis of multiple epigenetic marks in a single cell and requiring data from other cells to distinguish signal from experimental background noise in a single cell. This paper describes a method to reuse the same single cell for iterative epigenomic analyses. In this experimental method, cellular proteins are first anchored to a polyacrylamide polymer instead of crosslinking them to protein and DNA, alleviating structural bias. This critical step allows repeated experiments with the same single cell. Next, a random primer with a scaffold sequence for proximity ligation is annealed to the genomic DNA, and the genomic sequence is added to the primer by extension using a D

SUBMITTER: Ohnuki H 

PROVIDER: S-EPMC9376938 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature