Analysis of the anaerobic digestion metagenome under environmental stresses stimulating prophage induction.

ABSTRACT:

Background

The viral community has the potential to influence the structure of the microbiome and thus the yield of the anaerobic digestion process. However, the virome composition in anaerobic digestion is still under-investigated. A viral induction experiment was conducted on separate batches undergoing a series of DNA-damaging stresses, in order to coerce temperate viruses to enter the lytic cycle.

Results

The sequencing of the metagenome revealed a viral community almost entirely composed of tailed bacteriophages of the order Caudovirales. Following a binning procedure 1,092 viral and 120 prokaryotic genomes were reconstructed, 64 of which included an integrated prophage in their sequence. Clustering of coverage profiles revealed the presence of species, both viral and m

SUBMITTER: Rossi A 

PROVIDER: S-EPMC9377139 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature