Microphysiological System for High-Throughput Computer Vision Measurement of Microtissue Contraction.

ABSTRACT: The ability to measure microtissue contraction in vitro can provide important information when modeling cardiac, cardiovascular, respiratory, digestive, dermal, and skeletal tissues. However, measuring tissue contraction in vitro often requires the use of high number of cells per tissue construct along with time-consuming microscopy and image analysis. Here, we present an inexpensive, versatile, high-throughput platform to measure microtissue contraction in a 96-well plate configuration using one-step batch imaging. More specifically, optical fiber microprobes are embedded in microtissues, and contraction is measured as a function of the deflection of optical signals emitted from the end of the fibers. Signals can be measured from all the filled wells on the plate simultaneously using a di

SUBMITTER: Gracioso Martins AM 

PROVIDER: S-EPMC9377143 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature