Study on the biological mechanism of urolithin a on nasopharyngeal carcinoma in vitro.

ABSTRACT:

Context

Urolithin A (UroA) can inhibit the growth of many human cancer cells, but it has not be reported if UroA inhibits nasopharyngeal carcinoma (NPC) cells.

Objective

To explore the inhibitory effect of UroA on NPC and potential mechanism in vitro.

Materials and methods

RNA-sequencing-based mechanistic prediction was conducted by comparing KEGG enrichment of 40 μM UroA-treated for 24 h with untreated CNE2 cells. The untreated cells were selected as control. After NPC cells were treated with 20-60 μM UroA, proliferation, migration and invasion of were measured by colony formation, wound healing and transwell experiments. Apoptosis, mitochondrial membrane potential (MMP), reactive oxygen species (ROS) were measured by flow cytometry, Hoechst 33342, Rhodamine 1

SUBMITTER: Yang Y 

PROVIDER: S-EPMC9377270 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature