Classification of Developmental Toxicants in a Human iPSC Transcriptomics-Based Test.

ABSTRACT: Despite the progress made in developmental toxicology, there is a great need for in vitro tests that identify developmental toxicants in relation to human oral doses and blood concentrations. In the present study, we established the hiPSC-based UKK2 in vitro test and analyzed genome-wide expression profiles of 23 known teratogens and 16 non-teratogens. Compounds were analyzed at the maximal plasma concentration (C_max) and at 20-fold C_max for a 24 h incubation period in three independent experiments. Based on the 1000 probe sets with the highest variance and including information on cytotoxicity, penalized logistic regression with leave-one-out cross-validation was used to classify the compounds as test-positive or test-negative, reaching an area under th

SUBMITTER: Cherianidou A 

PROVIDER: S-EPMC9377669 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature