Rapid Detection of Beta-Lactamases Genes among Enterobacterales in Urine Samples by Using Real-Time PCR.

ABSTRACT: The objective of this study was to develop and evaluate newly improved, rapid, and reliable strategies based on real-time PCR to detect the most frequent beta-lactamase genes recorded in clinical Enterobacterales strains, particularly in Tunisia (bla_SHV12 , bla_TEM , bla_CTX-M-15 , bla_CTX-M-9 , bla_CMY-2 , bla_OXA-48 , bla_NDM-1 , and bla_IMP ) directly from the urine. Following the design of primers for a specific gene pool and their validation, a series of real-time PCR reactions were performed to detect these genes in 78 urine samples showing high antibiotic resistance after culture and susceptibility testing. Assays were applied to DNA extract

SUBMITTER: Yengui M 

PROVIDER: S-EPMC9377892 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature