Preparation, characterization, and osteogenic activity mechanism of casein phosphopeptide-calcium chelate.

ABSTRACT: Casein phosphopeptides (CPPs) are good at calcium-binding and intestinal calcium absorption, but there are few studies on the osteogenic activity of CPPs. In this study, the preparation of casein phosphopeptide calcium chelate (CPP-Ca) was optimized on the basis of previous studies, and its peptide-calcium chelating activity was characterized. Subsequently, the effects of CPP-Ca on the proliferation, differentiation, and mineralization of MC3T3-E1 cells were studied, and the differentiation mechanism of CPP-Ca on MC3T3-E1 cells was further elucidated by RNA sequencing (RNA-seq). The results showed that the calcium chelation rate of CPPs was 23.37%, and the calcium content of CPP-Ca reached 2.64 × 10⁵ mg/kg. The test results of Ultraviolet-Visible absorption spectroscopy (UV) and

SUBMITTER: Huang W 

PROVIDER: S-EPMC9378869 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature