Risk assessment for the spread of Candida sp. in dental chair unit waterlines using molecular techniques.

ABSTRACT:

Aims

The aim of this study was to evaluate the presence of yeasts in dental chair unit waterlines (DCUWLs) and to test their ability to form biofilms.

Materials and methods

Eighteen dental waterlines were analysed by culture in liquid Sabouraud in order to allow the quantification and the purification of isolated yeasts from their internal surfaces. All isolates were identified by standard laboratory procedures, including CHROMagar Candida medium for orientation, commercial yeast identification system Api Candida, MALDI-TOF MS and DNA sequencing. To evaluate their kinetics of antifungal susceptibility during different phases of biofilm formation, these yeasts were subjected to three antifungal agents.

Results

From the 18 DCUWLs studied, 10 were altered (55.56%). Eleve

SUBMITTER: Mazari W 

PROVIDER: S-EPMC9379022 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature