Non-invasive optogenetics with ultrasound-mediated gene delivery and red-light excitation.

ABSTRACT: Optogenetics has revolutionized the capability of controlling genetically modified neurons in vitro and in vivo and has become an indispensable neuroscience tool. Using light as a probe for selective neuronal activation or inhibition and as a means to read out neural activity has dramatically enhanced our understanding of complex neural circuits. However, a common limitation of optogenetic studies to date is their invasiveness and spatiotemporal range. Direct viral injections into the brain tissue along with implantation of optical fibers and recording electrodes can disrupt the neuronal circuitry and cause significant damage. Conventional approaches are spatially limited around the site of the direct injection and insufficient in examining large networks throughout the brain. Lastly, opto

SUBMITTER: Pouliopoulos AN 

PROVIDER: S-EPMC9379392 | biostudies-literature | 2022 Jul-Aug

REPOSITORIES: biostudies-literature