Multivalent interactions with RNA drive recruitment and dynamics in biomolecular condensates in Xenopus oocytes.

ABSTRACT: RNA localization and biomolecular condensate formation are key biological strategies for organizing the cytoplasm and generating cellular polarity. In Xenopus oocytes, RNAs required for germ layer patterning localize in biomolecular condensates, termed Localization bodies (L-bodies). Here, we have used an L-body RNA-binding protein, PTBP3, to test the role of RNA-protein interactions in regulating the biophysical characteristics of L-bodies in vivo and PTBP3-RNA condensates in vitro. Our results reveal that RNA-protein interactions drive recruitment of PTBP3 and localized RNA to L-bodies and that multivalent interactions tune the dynamics of the PTBP3 after localization. In a concentration-dependent manner, RNA becomes non-dynamic and interactions with the RNA determin

SUBMITTER: Cabral SE 

PROVIDER: S-EPMC9379569 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature