COVID-19 active case findings based on self-collected saliva samples with CRISPR-Cas12a detection.

ABSTRACT: COVID-19 is an infectious disease caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus affecting the world population. Early detection has become one of the most successful strategies to alleviate the epidemic and pandemic of this contagious coronavirus. Surveillance testing programs have been initiated in many countries worldwide to prevent the outbreak of COVID-19. In this study, we demonstrated that our previously established clustered regularly interspaced short palindromic repeats (CRISPR)-Cas12a-based assay could detect variants of concern during 2021 in Thailand, including Alpha, Beta, and Delta strains as well as Omicron strain in early 2022. In combination with the newly designed saliva collection funnel, we established a safe, simple, economical, and e

SUBMITTER: Chantaravisoot N 

PROVIDER: S-EPMC9379603 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature