Ageing and interferon gamma response drive the phenotype of neutrophils in the inflamed joint.

ABSTRACT:

Objective

Neutrophils are typically the most abundant leucocyte in arthritic synovial fluid. We sought to understand changes that occur in neutrophils as they migrate from blood to joint.

Methods

We performed RNA sequencing of neutrophils from healthy human blood, arthritic blood and arthritic synovial fluid, comparing transcriptional signatures with those from murine K/BxN serum transfer arthritis. We employed mass cytometry to quantify protein expression and sought to reproduce the synovial fluid phenotype ex vivo in cultured healthy blood neutrophils.

Results

Blood neutrophils from healthy donors and patients with active arthritis showed largely similar transcriptional signatures. By contrast, synovial fluid neutrophils exhibited more than 1600 differentially expre

SUBMITTER: Grieshaber-Bouyer R 

PROVIDER: S-EPMC9380120 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature