CellRegMap: a statistical framework for mapping context-specific regulatory variants using scRNA-seq.

ABSTRACT: Single-cell RNA sequencing (scRNA-seq) enables characterizing the cellular heterogeneity in human tissues. Recent technological advances have enabled the first population-scale scRNA-seq studies in hundreds of individuals, allowing to assay genetic effects with single-cell resolution. However, existing strategies to analyze these data remain based on principles established for the genetic analysis of bulk RNA-seq. In particular, current methods depend on a priori definitions of discrete cell types, and hence cannot assess allelic effects across subtle cell types and cell states. To address this, we propose the Cell Regulatory Map (CellRegMap), a statistical framework to test for and quantify genetic effects on gene expression in individual cells. CellRegMap provides a principled approach t

SUBMITTER: Cuomo ASE 

PROVIDER: S-EPMC9380406 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature