Genome-Wide Identification of the LexA-Mediated DNA Damage Response in Streptomyces venezuelae.

ABSTRACT: DNA damage triggers a widely conserved stress response in bacteria called the SOS response, which involves two key regulators, the activator RecA and the transcriptional repressor LexA. Despite the wide conservation of the SOS response, the number of genes controlled by LexA varies considerably between different organisms. The filamentous soil-dwelling bacteria of the genus Streptomyces contain LexA and RecA homologs, but their roles in Streptomyces have not been systematically studied. Here, we demonstrate that RecA and LexA are required for the survival of Streptomyces venezuelae during DNA-damaging conditions and for normal development during unperturbed growth. Monitoring the activity of a fluorescent recA promoter fusion and LexA protein levels revealed that the a

SUBMITTER: Stratton KJ 

PROVIDER: S-EPMC9380542 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature