Investigation and experimental validation of curcumin-related mechanisms against hepatocellular carcinoma based on network pharmacology.

ABSTRACT:

Objectives

To determine the potential molecular mechanisms underlying the therapeutic effect of curcumin on hepatocellular carcinoma (HCC) by network pharmacology and experimental in vitro validation.

Methods

The predictive targets of curcumin or HCC were collected from several databases. the identified overlapping targets were crossed with Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses using the Database for Annotation, Visualization, and Integrated Discovery (DAVID) platform. Two of the candidate pathways were selected to conduct an experimental verification. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide tetrazolium (MTT) assay was used to determine the effect of curcumin on the viability of HepG2 and LO2 cells. The apopto

SUBMITTER: Chen Y 

PROVIDER: S-EPMC9381327 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature