Identification of nosZ-expressing microorganisms consuming trace N₂O in microaerobic chemostat consortia dominated by an uncultured Burkholderiales.

ABSTRACT: Microorganisms possessing N₂O reductases (NosZ) are the only known environmental sink of N₂O. While oxygen inhibition of NosZ activity is widely known, environments where N₂O reduction occurs are often not devoid of O₂. However, little is known regarding N₂O reduction in microoxic systems. Here, 1.6-L chemostat cultures inoculated with activated sludge samples were sustained for ca. 100 days with low concentration (<2 ppmv) and feed rate (<1.44 µmoles h^-1) of N₂O, and the resulting microbial consortia were analyzed via quantitative PCR (qPCR) and metagenomic/metatranscriptomic analyses. Unintended but quantified intrusion of O₂ sustained dissolved oxygen concentration above 4 µM; however, complete N

SUBMITTER: Kim DD 

PROVIDER: S-EPMC9381517 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature