Generation of synthetic antibody fragments with optimal complementarity determining region lengths for Notch-1 recognition.

ABSTRACT: Synthetic antibodies have been engineered against a wide variety of antigens with desirable biophysical, biochemical, and pharmacological properties. Here, we describe the generation and characterization of synthetic antigen-binding fragments (Fabs) against Notch-1. Three single-framework synthetic Fab libraries, named S, F, and modified-F, were screened against the recombinant human Notch-1 extracellular domain using phage display. These libraries were built on a modified trastuzumab framework, containing two or four diversified complementarity-determining regions (CDRs) and different CDR diversity designs. In total, 12 Notch-1 Fabs were generated with 10 different CDRH3 lengths. These Fabs possessed a high affinity for Notch-1 (sub-nM to mid-nM K_Dapp values) and exhibited diff

SUBMITTER: Maruthachalam BV 

PROVIDER: S-EPMC9381698 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature