H3K27me3 at pericentromeric heterochromatin is a defining feature of the early mouse blastocyst.

ABSTRACT: Early mouse development is characterized by structural and epigenetic changes while cells progress towards differentiation. At blastocyst stage, the segregation of the three primordial lineages is accompanied by establishment of differential patterns of DNA methylation and post-translational modifications of histones, such as H3K27me3. Here, we analysed the dynamics of H3K27me3 at pericentromeric heterochromatin (PCH) during early development. We also followed the localization of EZH2 and BEND3, previously shown in ESCs to drive PRC2 to hypomethylated PCH. We show that the location of H3K27me3 at PCH, in addition to H3K9me3, is a defining feature of embryonic cells in vivo. Moreover, it may play an important role in structuring PCH and preserving genomic integrity at a time of globally rel

SUBMITTER: Pailles M 

PROVIDER: S-EPMC9381757 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature