Modelling immune cytotoxicity for cholangiocarcinoma with tumour-derived organoids and effector T cells.

ABSTRACT:

Background

Immunotherapy with immune checkpoint inhibitors (ICIs) is being explored to improve cholangiocarcinoma (CCA) therapy. However, it remains difficult to predict which ICI will be effective for individual patients. Therefore, the aim of this study is to develop a co-culture method with patient-derived CCA organoids and immune cells, which could represent anti-cancer immunity in vitro.

Methods

CCA organoids were co-cultured with peripheral blood mononuclear cells or T cells. Flow cytometry, time-lapse confocal imaging for apoptosis, and quantification of cytokeratin 19 fragment (CYFRA) release were applied to analyse organoid and immune cell behaviour. CCA organoids were also cultured in immune cell-conditioned media to analyse the effect of soluble factors.

Result

SUBMITTER: Zhou G 

PROVIDER: S-EPMC9381772 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature