Tyndall-effect-based colorimetric assay with colloidal silver nanoparticles for quantitative point-of-care detection of creatinine using a laser pointer pen and a smartphone.

ABSTRACT: Herein, this paper initially reports a new colorimetric Tyndall effect-inspired assay (TEA) for simple, low-cost, sensitive, specific, and point-of-care detection of creatinine (an important small biomolecule) by making use of silver nanoparticles (AgNPs) as model colloidal nanoprobes for visual light scattering signaling. The naked-eye TEA method adopts negatively-charged citrate-capped AgNPs (Cit-AgNPs) prepared by sodium citrate reduction. In the presence of alkaline conditions, the creatinine analyte can form carbanion/oxoanion amino tautomers which in turn crosslink with carboxylate groups on the Cit-AgNPs via a hydrogen bonding network to mediate the aggregation of such colloidal nanoprobes showing a significantly-enhanced TE signal that was created and quantified by a hand-he

SUBMITTER: Yuan K 

PROVIDER: S-EPMC9382227 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature