Discrimination of human papillomavirus genotypes using innovative technique nested-high resolution melting.

ABSTRACT: The prompt detection of human papillomavirus and discrimination of its genotypes by combining conventional methods in new molecular laboratories is essential to achieve the global call of eliminating cervical cancer. After predicting the melting temperature of an approximately 221 bp region of the L1 gene from different HPV genotypes by bioinformatics software, an innovative technique based on the nested- high resolution melting was designed with three approaches and using conventional PCR, qPCR, and diagnostic standards. HPV-positive samples identified by microarray along with diagnostic standards were evaluated by qPCR-HRM and discordant results were subjected to sequencing and analyzed in silico using reference types. In addition to screening for human papillomavirus, nested-qPCR-HRM is

SUBMITTER: Alirezaei M 

PROVIDER: S-EPMC9382607 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature