Dexamethasone suppresses the proliferation and migration of VSMCs by FAK in high glucose conditions.

ABSTRACT:

Background

High glucose conditions cause some changes in the vessels of diabetes through the signal transduction pathways. Dexamethasone and other corticosteroids have a wide range of biological effects in immunological events. In the present study, the effects of dexamethasone were investigated on the VSMC (vascular smooth muscle cell) proliferation, and migration based on the FAK gene and protein changes in high glucose conditions.

Methods and materials

The vascular smooth muscle cells were cultured in DMEM and were treated with dexamethasone (10^-7 M, 10^-6 M, and 10^-5 M) for 24, and 48 h in high glucose conditions. The cell viability was estimated by MTT method. The FAK gene expression levels and pFAK protein values were determined by RT-qP

SUBMITTER: Soleimani AA 

PROVIDER: S-EPMC9382766 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature