Identification and characterization of a new family of long satellite DNA, specific of true toads (Anura, Amphibia, Bufonidae).

ABSTRACT: Amphibians have some of the most variable genome sizes among vertebrates. Genome size variation has been attributed to repetitive and noncoding DNA, including satellite repeats, transposable elements, introns, and nuclear insertions of viral and organelle DNA. In vertebrates, satellite DNAs have been widely described in mammals, but few molecular studies have been carried out in amphibians. Here, we provide a detailed characterization of a new family of satellite DNA, present in all 15 examined species of the family Bufonidae. Southern-blot analysis and PCR reveal that this satellite is formed by monomers of 807 bp, is organized in tandem arrays, and has an AT-content of 57.4%. Phylogenetic analyses show that most clades exhibit species-specific variances, indicating that this satellite DN

SUBMITTER: Guzman K 

PROVIDER: S-EPMC9385698 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature