Hypoxia mimetics restore bone biomineralisation in hyperglycaemic environments.

ABSTRACT: Diabetic patients have an increased risk of fracture and an increased occurrence of impaired fracture healing. Diabetic and hyperglycaemic conditions have been shown to impair the cellular response to hypoxia, via an inhibited hypoxia inducible factor (HIF)-1α pathway. We investigated, using an in vitro hyperglycaemia bone tissue engineering model (and a multidisciplinary bone characterisation approach), the differing effects of glucose levels, hypoxia and chemicals known to stabilise HIF-1α (CoCl₂ and DMOG) on bone formation. Hypoxia (1% O₂) inhibited bone nodule formation and resulted in discrete biomineralisation as opposed to the mineralised extracellular collagen fibres found in normoxia (20% O₂). Unlike hypoxia, the use of hypoxia mimetics did not pre

SUBMITTER: Rezaei A 

PROVIDER: S-EPMC9385857 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature