Development of a Chemically Defined Medium for in vitro Expansion of Primary Bovine Satellite Cells.

ABSTRACT: The use of fetal bovine serum (FBS) in animal cell culture media is widely spread since it provides a broad spectrum of molecules that are known to support cell attachment and growth. However, the harvest and collection procedures of FBS raise ethical concerns and serum is an ill-defined and expensive component. This is especially problematic when it comes to regulatory approval for food applications like cultured meat. The aim of this study is to develop a chemically defined, cost efficient serum-free and animal-free medium that supports the attachment and expansion of bovine myoblasts while maintaining their differentiation capacity. Bovine satellite cells were harvested and isolated from a fresh sample of skeletal muscle tissue and cultured in planar systems. The efficacy of the tested

SUBMITTER: Kolkmann AM 

PROVIDER: S-EPMC9385969 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature