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High-content analysis of neuronal morphology in human iPSC-derived neurons.

ABSTRACT: We present a high-content analysis (HCA) protocol for monitoring the outgrowth capacity of human neurons derived from induced pluripotent stem cells (iPSCs). We describe steps to perform HCA imaging, followed by quantifying the morphology of dendrites and axons within a high-throughput system to evaluate neurons obtained through various differentiation approaches. This protocol can be used to screen for modulators of neuronal morphogenesis or neurotoxicity. The approach can be applied to patient-derived iPSCs to identify patient-specific defects and possible therapeutic strategies. For complete details on the use and execution of this protocol, please refer to Zink et al. (2020) and Inak et al. (2021). The protocol can be used in combination with Zink et al. (2022).

SUBMITTER: Lickfett S

PROVIDER: S-EPMC9386098 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature

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High-content analysis of neuronal morphology in human iPSC-derived neurons.

Lickfett Selene S Menacho Carmen C Zink Annika A Telugu Narasimha Swamy NS Beller Mathias M Diecke Sebastian S Cambridge Sidney S Prigione Alessandro A

STAR protocols 20220806 3

We present a high-content analysis (HCA) protocol for monitoring the outgrowth capacity of human neurons derived from induced pluripotent stem cells (iPSCs). We describe steps to perform HCA imaging, followed by quantifying the morphology of dendrites and axons within a high-throughput system to evaluate neurons obtained through various differentiation approaches. This protocol can be used to screen for modulators of neuronal morphogenesis or neurotoxicity. The approach can be applied to patient ...[more]

PMID: 35990743

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