Microbiome profiling of nasal extracellular vesicles in patients with allergic rhinitis.

ABSTRACT:

Background

Nasal microbiota is crucial for the pathogenesis of allergic rhinitis (AR), which has been reported to be different from that of healthy individuals. However, no study has investigated the microbiota in nasal extracellular vesicles (EVs). We aimed to compare the microbiome composition and diversity in EVs between AR patients and healthy controls (HCs) and reveal the potential metabolic mechanisms in AR.

Methods

Eosinophil counts and serum immunoglobulin E (IgE) levels were measured in patients with AR (n = 20) and HCs (n = 19). Nasal EVs were identified using transmission electron microscopy and flow cytometry. 16S rRNA sequencing was used to profile the microbial communities. Alpha and beta diversities were analyzed to determine microbial diversity. Taxonomic abu

SUBMITTER: Chiang TY 

PROVIDER: S-EPMC9386106 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature