Development of a new TaqMan-based real-time RT-PCR assay for the specific detection of bovine kobuvirus.

ABSTRACT: Bovine kobuvirus (BKV) is a novel kobuvirus considered to be closely related to calf diarrhea and has become a worldwide epidemic. Currently, the BKV lacks an efficient and convenient detection method to assist the research on BKV prevalence. In this study, a new and specific TaqMan-based real-time RT-PCR for the detection of BKV was developed using the conserved region of the 3D gene. The assay was highly specific for BKV, without cross-amplification with other non-targeted pathogens. The limit of detection of this assay was 10² copies. Standard curves showed a strong linear correlation from 10² to 10⁶ copies of BKV standard RNA per reaction, and the parameters revealed as a slope of -3.54, efficiency of 91.64%, and regression coefficients (R²)

SUBMITTER: Liu Y 

PROVIDER: S-EPMC9386250 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature