Development of a rapid homogeneous immunoassay for detection of rotavirus in stool samples.

ABSTRACT: Rotavirus is the main pathogen causing acute viral gastroenteritis. Accurate and rapid diagnosis of rotavirus infection is important to determine appropriate treatment, prevention of unnecessary antibiotics use and control of infection spread. In this study, we established a rapid, accurate, and sensitive amplified luminescent proximity homogeneous assay linked immunosorbent assay (AlphaLISA) for detecting rotavirus and evaluated its efficacy in human stool samples. Our results demonstrated that the sensitivity of AlphaLISA (5^-8) significantly exceeded that of the immunochromatographic assay (ICA, 5^-4) for rotavirus antigen detection. The intra-assay and inter-assay coefficients of variation were 2.99-3.85% and 5.27-6.51%, respectively. Furthermore, AlphaLISA was spec

SUBMITTER: Wang Y 

PROVIDER: S-EPMC9386352 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature