The conserved Mediator subunit cyclin C (CCNC) is required for brown adipocyte development and lipid accumulation.

ABSTRACT:

Objective

Cyclin C (CCNC) is the most conserved subunit of the Mediator complex, which is an important transcription cofactor. Recently, we have found that CCNC facilitates brown adipogenesis in vitro by activating C/EBPα-dependent transcription. However, the role of CCNC in brown adipose tissue (BAT) in vivo remains unclear.

Methods

We generated conditional knock-out mice by crossing Ccnc^flox/flox mice with Myf5^Cre, Ucp1^Cre or Adipoq^Cre transgenic mice to investigate the role of CCNC in BAT development and function. We applied glucose and insulin tolerance test, cold exposure and indirect calorimetry to capture the physiological phenotypes and used immunostaining, immunoblotting, qRT-PCR, RNA-seq and cell culture to elucidate the

SUBMITTER: Song Z 

PROVIDER: S-EPMC9386464 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature