Same-day confirmation of infection and antimicrobial susceptibility profiling using flow cytometry.

ABSTRACT:

Background

The annual mortality burden of antimicrobial resistant infections exceeds 1.27 million/year. With serious infections, every hour without effective antimicrobial therapy results in a 6.7% increased risk of death. New technology that delivers actionable pathology results in clinically-relevant timeframes is an urgent priority. We present the development and validation of an acoustic-enhanced flow cytometric (AFC) workflow that provides same-day confirmation of infection and antimicrobial susceptibility, using peritoneal dialysis (PD)-associated peritonitis as a demonstrative example.

Methods

In this cohort study, we analysed peritoneal dialysis effluent specimens using AFC to confirm the presence of infection and antimicrobial susceptibility of identified organisms.

SUBMITTER: Mulroney K 

PROVIDER: S-EPMC9386725 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature