Development of an Automatable Affinity Purification Process for DNA-Encoded Chemistry.

ABSTRACT: DNA-encoded library technologies require high-throughput, compatible, and well automatable platforms for chemistry development, building block rehearsal, and library synthesis. An affinity-based process using Watson-Crick interactions was developed that enables purification of DNA-tagged compounds from complex reaction mixtures. The purification relies on a single-stranded DNA-oligonucleotide, called capture strand, which was covalently coupled to an agarose matrix and to which a DNA-compound conjugate from a DNA-encoded library (DEL) reaction can be reversibly annealed to. The thus-formed DNA duplex tolerated surprisingly stringent washing conditions with multiple solvents to remove excess reactants and reagents. The tolerated solvents included aqueous buffers, aqueous EDTA solutio

SUBMITTER: Gotte K 

PROVIDER: S-EPMC9386796 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature