MiR-19b-3p regulated by BC002059/ABHD10 axis promotes cell apoptosis in myocardial infarction.

ABSTRACT:

Background

Recently, microRNAs (miRNAs), have been extensively investigated in diseases. The upregulated expression of miR-19b-3p has been validated in patients with hypertrophic cardiomyopathy. Nonetheless, it regulatory mechanism in myocardial infarction (MI) is still unclear.

Purpose

This research aimed to investigate the role and molecular regulation mechanism of miR-19b-3p in MI.

Methods

QRT-PCR and western blot assays measured RNA and protein expression. Cell apoptosis were tested by flow cytometry and TUNEL assays. Cell viability was measured by trypan blue staining method. RIP and luciferase report assays examined gene interaction. The assays were performed under hypoxia condition.

Results

MiR-19b-3p was highly expressed in myocardial cell line H9C2, pr

SUBMITTER: Liao B 

PROVIDER: S-EPMC9386969 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature