MiR-215-5p regulates osteoporosis development and osteogenic differentiation by targeting XIAP.

ABSTRACT:

Background

Osteoporosis (OP) is a metabolic disease that involves microstructure destruction and fracture damage. The present study probed into the significance of miR-215-5p in OP progression.

Methods

Serum samples were collected from surgical patients and healthy controls. qRT-PCR analysis was utilized to determine the miR-215-5p level in clinical samples and human bone mesenchymal stem cells (hBMSCs) induced by β-glycerol phosphate. A dual luciferase reporter assay was exploited to examine the targeted relationship between miR-215-5p and XIAP. The mineralization and calcium deposition of hBMSCs were assessed by detection of ALP activity, Alizarin red staining, and osteoblast marker expression. Protein expression was determined by western blot analysis.

Results

MiR-

SUBMITTER: Yin Z 

PROVIDER: S-EPMC9387055 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature