SARS-CoV-2 triggers complement activation through interactions with heparan sulfate.

ABSTRACT:

Objectives

To determine whether SARS-CoV-2 can trigger complement activation, the pathways that are involved and the functional significance of the resultant effect.

Methods

SARS-CoV-2 was inoculated into a human lepirudin-anticoagulated whole blood model, which contains a full repertoire of complement factors and leukocytes that express complement receptors. Complement activation was determined by measuring C5a production with an ELISA, and pretreatment with specific inhibitors was used to identify the pathways involved. The functional significance of this was then assessed by measuring markers of C5a signalling including leukocyte C5aR1 internalisation and CD11b upregulation with flow cytometry.

Results

SARS-CoV-2 inoculation in this whole blood model caused progres

SUBMITTER: Lo MW 

PROVIDER: S-EPMC9387400 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature