Characterization of Aldosterone-producing Cell Cluster (APCC) at Single-cell Resolution.

ABSTRACT:

Context

The adrenal cortex consists of zona glomerulosa (ZG), fasciculata (ZF), and reticularis. Aldosterone-producing cell clusters (APCCs) that strongly express aldosterone synthase (CYP11B2) are frequently found in adult adrenals and harbor somatic mutations that are also detected in aldosterone-producing adenomas (APAs). Primary aldosteronism is mainly caused by APAs or idiopathic hyperaldosteronism (IHA). We presume that APCCs are causing IHA and are precursors of APAs. However, the gene expression characteristics and especially the development of APCCs are not well understood.

Objective

This study aimed to analyze the transcriptome of APCCs at single-cell resolution and infer the developmental trajectory.

Methods

Single-cell RNA sequencing (scRNA-seq) of 2 adult

SUBMITTER: Iwahashi N 

PROVIDER: S-EPMC9387688 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature