The physiological cargo adaptor of kinesin-2 functions as an evolutionary conserved lockpick.

ABSTRACT: Specific recognition of cellular cargo and efficient transport to its correct intracellular destination is an infrastructural challenge faced by most eukaryotic cells. This remarkable deed is accomplished by processive motor proteins that are subject to robust regulatory mechanisms. The first level of regulation entails the ability of the motor to suppress its own activity. This autoinhibition is eventually relieved by specific cargo binding. To better understand the role of the cargo during motor activation, we dissected the activation mechanism of the ciliary homodimeric kinesin-2 from Caenorhabditis elegans by its physiological cargo. In functional reconstitution assays, we identified two cargo adaptor proteins that together are necessary and sufficient to allosterically activate

SUBMITTER: Cleetus A 

PROVIDER: S-EPMC9388150 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature